四川泡菜产生物胺细菌的筛选及产胺能力验证-《中国调味品》

文章信息/Info

Title:: Screening of Amine-producing Bacteria From Sichuan Paocai and Verification of Amine Production Ability

摘要:: 以四川工业泡菜和家庭泡菜为研究对象，分析其一般理化性质、微生物数量及生物胺含量，并对产生物胺细菌进行筛选及产胺能力验证。结果表明，四川工业泡菜的盐度较高，为11.15±0.11mg/kg，菌落总数及乳酸菌总数较低，家庭泡菜的pH较低，总酸含量较高，分为3.55±0.04和1.30±0.00g/100g。腐胺、尸胺、组胺、酪胺为四川泡菜主要的生物胺，含量均超过100 mg/kg，且在四川工业泡菜中，尸胺含量高达349.43±13.23mg/kg。从四川泡菜中筛选出11株产生物胺的细菌，经鉴定分别为解鸟氨酸拉乌尔菌（Raoultella ornithinolytica）、弗氏柠檬酸杆菌（Citrobacter freundii）、特基拉芽孢杆菌（Bacillus tequilensis）、阿耶波多氏芽孢杆菌（Bacillus aryabhattai），其中R.ornithinolytica含有赖氨酸脱羧酶cacd基因和组氨酸脱羧酶hdc基因，且回接培养基验证了其具有尸胺和组胺形成能力，48h尸胺形成量高达766.67±2.17mg/L，可能是四川工业泡菜尸胺含量较高的原因。论文结果可为四川泡菜生物胺含量的调控提供参考。

Abstract:: Taking Sichuan industrial Paocai and family Paocai as research objects, the general physical and chemical properties, microbial quantity and biogenic amine content were analyzed, and the production of amine-producing bacteria and the ability to produce amines were verified. The results showed that the salinity of Sichuan Paocai was higher, 11.15±0.11mg/kg, the total number of colonies and the total number of lactic acid bacteria were lower, the pH of family Paocai was lower, and the total acid content was higher, which was divided into 3.55±0.04 and 1.30±0.00g/100g. Putrescine, cadaverine, histamine and tyramine are the main biogenic amines of Sichuan Paocai, and their contents are more than 100 mg/kg. In Sichuan industrial Paocai, the cadaverine content is as high as 349.43±13.23 mg/kg. Eleven strains of amine-producing bacteria were screened from Sichuan Paocai, and identified as Raoultella ornithinolytica, Citrobacter freundii, Bacillus tequilensis, and Bacillus aryabhattai, Detection of R. ornithinolytica containing lysine decarboxylase cacd gene and histidine decarboxylase hdc gene, It was confirmed in the retort medium that it had cadaverine and histamine formation ability. The formation of cadaverine in 48h was as high as 766.67±2.17mg/L,which may be the reason for the higher content of cadaverine in Sichuan industrial Paocai. The results of the paper can provide reference for the regulation of the content of biogenic amines in Sichuan Paocai.