[1]李佳欢,都新荣,王若瑾,等.真姬菇生产废料不同溶剂提取物抗氧化活性研究[J].中国调味品,2019,(02):33-38.
 Study on Antioxidant Activity of Different Solvent Extracts of Hypsizygus marmoreus Waste[J].CHINA CONDIMENT,2019,(02):33-38.
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真姬菇生产废料不同溶剂提取物抗氧化活性研究()
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《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2019年02期
页码:
33-38
栏目:
出版日期:
2019-02-20

文章信息/Info

Title:
Study on Antioxidant Activity of Different Solvent Extracts of Hypsizygus marmoreus Waste
作者:
李佳欢都新荣王若瑾金文松胡开辉
文献标志码:
A
摘要:
通过ABTS法、DPPH法以及亚铁还原能力测定等方法,比较真姬菇生产废料(菇脚、小菇)与成品菇在不同溶剂(水、甲醇、正丁醇、丙酮)中提取物的抗氧化活性,并分析抗氧化活性与总酚含量的关系。结果表明,三种样品的水提取物清除自由基能力及总还原能力最好,其次是甲醇提取物,丙酮与正丁醇效果较差。同时通过福林-酚比色法检测得到了各溶剂提取物中的总酚含量,发现样品总酚含量与其抗氧化活性之间具有良好的相关性。真姬菇菇脚、小菇、成品菇水提物ABTS法的EC50值分别为1.193、1.109和1.220mg/mL,无显著差异。说明真姬菇菇脚、小菇可作为天然抗氧化剂的提取原料应用于食品科学领域。
Abstract:
The antioxidant activities of four solvents (water, methanol, n-butanol and acetone) extracts from waste of H.marmoreus(stembase and small H.marmoreus)and finished mushroom were studied by determination of ABTS, DPPH and ferrous reduction capacity. Then, the relationship between antioxidant activity and total polyphenol content was analyzed. The results showed that the highest ABTS radical scavenging activities, DPPH radical scavenging activities and ferrous reduction capacity were found for water fraction, followed by methanol extract, and the effect of acetone and n-butanol was poor. At the same time, the total phenol content of each solvent extract was measured by Folin-Ciocalteu method. It was found that there was a good correlation between the total phenol content and the antioxidant activity. The ABTS radical scavenging activities of the water extract from mushroom stembase, small mushroom and finished mushroom were 1.193, 1.109 and 1.220mg/mL,respectively, without significantly differ between group. It indicated that the H.marmoreus stembase and small H.marmoreus can be used as raw material for natural antioxidant in the field of food science.
更新日期/Last Update: 2019-03-18