[1]石峰,熊华斌,李思敏等.基于真空冷冻干燥的响应面优化超声提取青杨梅中抗氧化物质及活性分析[J].中国调味品,2021,46(4):43-49.
 Optimization of Ultrasonic Extraction Technology by Response Surface Methodology and Activity Analysis of Antioxidants from Myrica Adenophora Hance Based on Vacuum Freeze Drying[J].CHINA CONDIMENT,2021,46(4):43-49.
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基于真空冷冻干燥的响应面优化超声提取青杨梅中抗氧化物质及活性分析()
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《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
46
期数:
2021年4期
页码:
43-49
栏目:
出版日期:
2021-04-09

文章信息/Info

Title:
Optimization of Ultrasonic Extraction Technology by Response Surface Methodology and Activity Analysis of Antioxidants from Myrica Adenophora Hance Based on Vacuum Freeze Drying
作者:
石峰熊华斌李思敏等
文献标志码:
A
摘要:
本文以云南野生青杨梅为原料,在真空冷冻干燥获得活性物质最大程度保留的基础上,通过单因素实验和响应面优化设计研究了云南野生青杨梅抗氧化物质的提取及其抗氧化活性。结果表明,1:25g/ml的固液比和体积分数为60%的乙醇对青杨梅抗氧化物质的提取效果最佳。通过响应面优化得到的最佳条件是超声功率573.29W?、超声时间27.63min、提取温度50℃,预测最优DPPH·清除率为52.02%。经过实验得到了提取物与DPPH·反应30s时的清除率达到52.14%,与预测值十分接近,相差仅0.12%。这说明真空冷冻干燥下的响应面法优化超声提取云南野生青杨梅中抗氧化物质工艺是可行的,可为青杨梅的开发、利用提供理论和技术参考。
Abstract:
In this paper, the extraction of antioxidative substances and the antioxidative activity of wild Asparagus angustifolia in Yunnan were carried out by using single factor experiment and response surface optimization design on the basis of maximizing the retention of active substances in the vacuum freeze drying in Yunnan. The results showed that 1:25g/ml solid-liquid ratio and 60% volume fraction of ethanol had the best extraction effect on the antioxidative substances of Myrica rubra. The optimal conditions obtained by response surface optimization are ultrasonic power 573.29W?, ultrasonic time 27.63min, extraction temperature 50℃, and the optimal DPPH·clearance rate is predicted to be 52.02%. After the experiment, the clearance rate of the extract with DPPH·30s reached 52.14%, which was very close to the predicted value, and the difference was only 0.12%. This shows that the response surface method under vacuum freeze-drying is feasible to optimize the process of ultrasonic extraction of antioxidant substances from wild aspen in Yunnan, and it can provide theoretical and technical references for the development and utilization of aspen.
更新日期/Last Update: 2021-04-09