[1]刘兰,陈雅楠,范宝莉,等.高效液相色谱法测定大蒜愈伤组织中大蒜素的含量[J].中国调味品,2019,(02):1-4.
Determination of Allicin in Garlic Callus by High Performance Liquid Chromatography[J].CHINA CONDIMENT,2019,(02):1-4.
点击复制
高效液相色谱法测定大蒜愈伤组织中大蒜素的含量()
《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]
- 卷:
-
- 期数:
-
2019年02期
- 页码:
-
1-4
- 栏目:
-
- 出版日期:
-
2019-02-20
文章信息/Info
- Title:
-
Determination of Allicin in Garlic Callus by High Performance Liquid Chromatography
- 作者:
-
刘兰; 陈雅楠; 范宝莉; 王振英; 尚云涛
-
- 文献标志码:
-
A
- 摘要:
-
建立了高效液相色谱法测定大蒜愈伤组织中大蒜素含量的分析方法。色谱柱Eclipse Plus C18,5μm,4.6×250 mm,柱温35℃,流动相为甲醇-水含0.2%甲酸(80:20),流速1.0 ml/min,二极管阵列检测器检测波长240 nm,进样量50 μL。结果显示,大蒜素在0.30~4.57 μg/ml范围内线性关系良好,相关系数R2=0.999,加标回收率为95% ~ 102%。该方法灵敏度高,重现性好,操作简便,适用于大蒜愈伤组织中大蒜素含量的检测。
- Abstract:
-
Develop a method for the determination of allicin in garlic callus by high performance liquid chromatography (HPLC). The conditions of HPLC are taken as follows: the chromatography column is Eclipse Plus C18(5μm,4.6×250 mm℃, the mobile phase methanol : water (0.2 formic acid) is 80:20(V/V), the flow rate is 1.0 ml/min, the DAD detector wavelength is 240 nm, and the sample injection volume is 50 μL. The results show that the content of allicin is in the range of 0.30 ~ 4.57 μg/ml, it has a good linear relationship, and the correlation coefficient is 0.999, and the average recovery of allicin is 95% ~ 102%. This method is accurate, reliable, simple and efficient for the detection of allicin in garlic callus
更新日期/Last Update:
2019-03-18