大孔树脂纯化仙草黄酮及成分测定-《中国调味品》

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Title:: Purification and etermination of Mesona Blume Flavonoids by Macroporous resin

摘要:: 为研究仙草黄酮纯化工艺及其主要组分的结构，以仙草为原料，采用大孔树脂（HPD-100）、羟丙基葡聚糖凝胶（Sephadex LH-20）分别对仙草黄酮进行柱层析纯化和组分分离，利用HPLC、1H-NMR、13C-NMR对分离得到的化合物进行分析和结构鉴定。结果表明，HPD-100大孔树脂纯化仙草黄酮效果良好，动态吸附工艺参数为：上样量为3 BV，上样流速为3 BV/h，洗脱剂乙醇浓度为60 %，洗脱流速为6 BV /h。通过Sephadex LH-20柱层析分离得到两种黄酮类化合物，HPLC分析表明两者纯度皆在90 %以上，经鉴定为迷迭香酸（rosmarinic acid，MBF1）与紫云英苷（astragaloside，MBF2）。本研究可为仙草资源的高值化利用提供一定的理论依据。

Abstract:: In order to study the purification process and main components of Flavonoids from the Mesona Blume., the constituents of the extract were separated and purified by macroporous resin (HPD-100) and hydroxypropyl dextran gel (Sephadex LH-20). The main components were separated and analyzed by HPLC, 1H-NMR and 13C-NMR. The results showed that HPD-100 macroporous resin had a good purification effect. The dynamic adsorption parameters were as follows: the amount of sample was 3 BV, the flow rate of sample was 3 bv / h, the concentration of eluent ethanol was 60 %, and the flow rate of eluent was 6 bv / h. Two flavonoids were separated by Sephadex LH-20 column chromatography. HPLC analysis showed that the purity of both flavonoids was over 90 %. They were identified as rosmarinic acid (MBF1) and astragaloside (MBF2). This study can provide a theoretical basis for the high value utilization of Mesona Blume.o resources.