动物蛋白水解酶法制备南极磷虾多肽及其抗氧化活性-《中国调味品》

文章信息/Info

Title:: Preparation and Antioxidant Activity of Antarctic Krill (Euphausia superba) Peptides by Animal Proteolytic Enzyme

摘要:: 本文以南极磷虾虾糜和蛋白为原料，通过动物蛋白水解酶制备南极磷虾多肽，研究了酶解的工艺参数，分析了多肽的分子量分布，并评价了多肽的抗氧化活性。结果表明：通过动物蛋白水解酶对南极磷虾虾糜和蛋白进行水解，最优的酶解温度为50°C，pH为8.0，加酶量为2000 U/g，酶解时间为3 h。此条件下，得到的两种南极磷虾多肽分子量范围为311~2963 Da，且此两种南极磷虾多肽具有一定的抗氧化能力，能清除羟基自由基和还原铁离子。

Abstract:: In this paper, Antarctic krill peptides were prepared by animal proteolytic enzymes using Antarctic krill surimi and protein as raw materials. The parameters of enzymatic hydrolysis were studied, the molecular weight distribution range of the peptide was analyzed, and the antioxidant activity of the peptide was evaluated. The results indicated that the optimized hydrolyzed temperature was 50°C, the pH was 8.0, the added amount of enzyme was 2000 U/g, and the hydrolyzed time was 3 h. Under the above conditions, peptides from Antarctic krill surimi and protein have the molecular weights ranging from 311 Da to 2963 Da. These two peptides also had certain hydroxyl radical-scavenging ability and ferric-reducing ability.