一种利用玉米胚粕与玉米黄浆制作新型培养基发酵生产谷胱甘肽的方法-《中国调味品》

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Title:: The invention relates to a method of producing glutathione by fermentation of maize embryo meal and maize yellow pulp

摘要:: 为研究玉米胚粕与玉米黄浆新型培养基发酵生产谷胱甘肽的能力。选取糖化且经过三效浓缩后的玉米胚粕与玉米黄浆为原料，制成新型酵母菌发酵培养基,对酿酒酵母S.cerevisiae L5267进行发酵。通过单因素试验与正交试验选取最优条件。利用琥珀酸二酯磺酸钠与异辛烷形成的反胶束体系对谷胱甘肽进行反胶束萃取，萃取后检测萃取液中谷胱甘肽含量。结果表明，玉米胚粕与玉米黄浆糖化液DE值为92：培养基配比；L-cys 0.4 g、MgSO4 0.125 g、肌醇 0. 0375 g、KCL 1.8 g、糖化液100 g、蒸馏水150 g：此条件下，按照接种量7%,发酵温度30℃，发酵时间23h进行发酵。发酵后经琥珀酸二脂磺酸钠与异辛烷反胶团萃取后，最终谷胱甘肽的含量可达到10.01mg/g，在经过纳滤膜分离设备与低温冷冻干燥后得到谷胱甘肽固体成品。

Abstract:: In order to study the fermentation capacity of glutathione from corn germ meal and corn steep liquor.The saccharified corn germ meal and corn steep liquor were selected as the raw materials to make a new yeast fermentation medium for the fermentation of S.cerevisiae L5267 .The optimal conditions were selected by single factor test and orthogonal test.Glutathione was extracted by reverse micelle system formed by Sodium diethylhexyl sulfosuccinate and isooctane, glutathione was obtained by ultrafiltration and cryogenic freeze-drying and its content was calculated .theresultsshowthat,when the DE value of corn germ meal and corn steep liquor was 9 2;Medium ratio:L-cys 0.4 g,MgSO4 0.125 g,Inositolum 0.0375 g ,KCL 1.8 g,saccharification solution 100 g,distilledwater 150 g;Under these conditions, fermentation was conducted according to the inoculation amount of 7%, fermentation temperature of 30℃ and fermentation time of 23h.After fermentation, the final glutathione content reached 20.41mg/g after extraction with sodium succinic disulphonate and isooctane.Glutathione solid products were obtained by nanofiltration membrane separation equipment and cryogenic freeze-drying