The yields of kiwi seed oils of main cultivars such as Jinyan, Hongyang, Hongshi No. 2, Jinshi No. 1, Hayward, Xuxiang and New Zealand in Sichuan areas are analyzed and compared, the fatty acid composition and squalene relative content of 7 varieties are analyzed by GC-MS combined with PCA. The results show that the yield of Hayward kiwi seed oil is the highest (28.28%), and the unsaturated fatty acids such as linolenic acid, oleic acid and linoleic acid are the main fatty acids in kiwi seed oils (85.7%~87.1%). The fatty acids of linolenic acid, hexadecanoic acid and octadecanoic acid have great differences among different cultivars, but the fatty acid composition of different kiwi seed oils has little difference. The relative content of squalene in different varieties of seed oils ranges from 1.01% to 1.72%, Jinyan has the highest content (1.72%) and Jinshi No. 1 has the lowest content (1.01%).